.. _metabc02:

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Metabarcoding online seminar - 2022: Part 2
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The second part of the seminar focusses on specific options for amplicon sequencing data processing. All discussed options are part of the `dadasnake <https://github.com/a-h-b/dadasnake>`_ pipeline. The topics are:

  * dadasnake – aims and realization
  * dadasnake and other workflows: options in detail

    * re-sequencing
    * steps
    * primer removal
    * trimming and QC filtering
    * downsampling
    * error models
    * ASV generation
    * paired-ends assembly
    * chimera removal
    * taxonomic annotation
    * functional prediction
 
  * snakemake
  * dadasnake - what to do if it doesn’t work

You can download the slides here: :download:`slides part 2 <_static/220602_Metabarcoding_p2.pdf>`. 

A video is available on the University of Amsterdam's `media site <https://video.uva.nl/media/Metabarcoding+-+part+2/0_1sfohxxh>`_.