Metabarcoding online seminar - 2022: Part 2

The second part of the seminar focusses on specific options for amplicon sequencing data processing. All discussed options are part of the dadasnake pipeline. The topics are:

• dadasnake – aims and realization

• dadasnake and other workflows: options in detail

  • re-sequencing

  • steps

  • primer removal

  • trimming and QC filtering

  • downsampling

  • error models

  • ASV generation

  • paired-ends assembly

  • chimera removal

  • taxonomic annotation

  • functional prediction

• snakemake

• dadasnake - what to do if it doesn’t work

You can download the slides here: slides part 2.

A video is available on the University of Amsterdam’s media site.