Metabarcoding online seminar - 2022: Part 2

The second part of the seminar focusses on specific options for amplicon sequencing data processing. All discussed options are part of the dadasnake pipeline. The topics are:

  • dadasnake – aims and realization

  • dadasnake and other workflows: options in detail

    • re-sequencing

    • steps

    • primer removal

    • trimming and QC filtering

    • downsampling

    • error models

    • ASV generation

    • paired-ends assembly

    • chimera removal

    • taxonomic annotation

    • functional prediction

  • snakemake

  • dadasnake - what to do if it doesn’t work

You can download the slides here: slides part 2.

A video is available on the University of Amsterdam’s media site.